mouse anti p38 monoclonal antibody  (Proteintech)

Journal: eLife

Article Title: Nicotine enhances the stemness and tumorigenicity in intestinal stem cells via Hippo-YAP/TAZ and Notch signal pathway

doi: 10.7554/eLife.95267

Figure Lengend Snippet:

Article Snippet: Antibody , mouse monoclonal anti-phospho-p38 , Santa Cruz , #sc-166182 , WB (1:100).

Techniques:

Journal: eLife

Article Title: Nicotine enhances the stemness and tumorigenicity in intestinal stem cells via Hippo-YAP/TAZ and Notch signal pathway

doi: 10.7554/eLife.95267

Figure Lengend Snippet:

Article Snippet: Antibody , mouse monoclonal anti-p38 , Santa Cruz , #sc-81621 , WB (1:200).

Techniques:

Journal: Oncology letters

Article Title: Co‑expression of SLC20A1 and ALDH1A3 is associated with poor prognosis, and SLC20A1 is required for the survival of ALDH1‑positive pancreatic cancer stem cells.

doi: 10.3892/ol.2024.14558

Figure Lengend Snippet: Figure 4. Western blot analysis of proteins associated with p38, JNK, p44/42 and Akt signaling following SLC20A1 knockdown in PANC‑1 and MIA‑PaCa‑2 cells. SLC20A1 was knocked down in PANC‑1 and MIA‑PaCa‑2 cells by incubation with SLC20A1 siRNA (or NC siRNA) in a two‑dimensional monolayer culture for 48 h, and the subsequent three‑dimensional tumorspheres were cultured for 72 h (PANC‑1) or 24 h (MIA‑PaCa‑2). (A) Representative western blot images. Relative SLC20A1 mRNA expression following transfection with SLC20A1 siRNA or NC siRNA in (B) PANC‑1 and (C) MIA‑PACA‑2 cells. Levels of p‑p38 in (D) PANC‑1 and (E) MIA‑PaCa‑2 cells. Levels of p‑p44/42 in (F) PANC‑1 and (G) MIA‑PaCa‑2 cells. Level of p‑JNK in (H) PANC‑1 and (I) MIA‑PaCa‑2 cells. Level of p‑AKT S473 in (J) PANC‑1 and (K) MIA‑PaCa‑2 cells. The molecular weight of phosphorylated proteins and proteins is shown on the right of the blot images. (D‑K) Evaluation of the ratio of phosphorylated protein/total protein. *P<0.05, **P<0.01 vs. NC; unpaired Student's t‑test. SLC20A1, solute carrier family 20 member 1; siRNA, small interfering RNA; NC, negative control; KD, knockdown; p‑, phosphorylated.

Article Snippet: The primary antibodies were as follows: mouse anti‐p‐p38 monoclonal antibody (mAb) (cat. no. 9216s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐p38 polyclonal antibody (pAb) (cat. no. 9212s; Cell Signaling Technology, Inc. Danvers, MA, USA; 1:3,000); rabbit anti‐phosphorylated (p)‐c‐Jun N‐terminal Kinase (JNK) pAb (cat. no. 9251s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐JNK pAb (cat. no. 9252s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐p‐p44/p42 pAb (cat. no. 9101s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐p44/p42 pAb (cat. no. 9102s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐p‐Akt S473 mAb (cat. no. 4060s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐Akt mAb (cat. no. 2938s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐ALDH1A1 mAb (cat. no. ab52492; Abcam; 1:5,000); rabbit anti‐ALDH1A3 pAb (cat. no. PA5‐29188; Thermo Fisher Scientific, Inc.; 1:5,000); and mouse anti‐β‐actin mAb (cat. no. 60008‐1‐Ig; ProteinTech Group, Inc.; 1:10,000).

Techniques: Western Blot, Knockdown, Incubation, Cell Culture, Expressing, Transfection, Molecular Weight, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: Co‑expression of SLC20A1 and ALDH1A3 is associated with poor prognosis, and SLC20A1 is required for the survival of ALDH1‑positive pancreatic cancer stem cells

doi: 10.3892/ol.2024.14558

Figure Lengend Snippet: Western blot analysis of proteins associated with p38, JNK, p44/42 and Akt signaling following SLC20A1 knockdown in PANC-1 and MIA-PaCa-2 cells. SLC20A1 was knocked down in PANC-1 and MIA-PaCa-2 cells by incubation with SLC20A1 siRNA (or NC siRNA) in a two-dimensional monolayer culture for 48 h, and the subsequent three-dimensional tumorspheres were cultured for 72 h (PANC-1) or 24 h (MIA-PaCa-2). (A) Representative western blot images. Relative SLC20A1 mRNA expression following transfection with SLC20A1 siRNA or NC siRNA in (B) PANC-1 and (C) MIA-PACA-2 cells. Levels of p-p38 in (D) PANC-1 and (E) MIA-PaCa-2 cells. Levels of p-p44/42 in (F) PANC-1 and (G) MIA-PaCa-2 cells. Level of p-JNK in (H) PANC-1 and (I) MIA-PaCa-2 cells. Level of p-AKT S473 in (J) PANC-1 and (K) MIA-PaCa-2 cells. The molecular weight of phosphorylated proteins and proteins is shown on the right of the blot images. (D-K) Evaluation of the ratio of phosphorylated protein/total protein. *P<0.05, **P<0.01 vs. NC; unpaired Student's t-test. SLC20A1 , solute carrier family 20 member 1; siRNA, small interfering RNA; NC, negative control; KD, knockdown; p-, phosphorylated.

Article Snippet: The primary antibodies were as follows: mouse anti-p-p38 monoclonal antibody (mAb) (cat. no. 9216s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-p38 polyclonal antibody (pAb) (cat. no. 9212s; Cell Signaling Technology, Inc. Danvers, MA, USA; 1:3,000); rabbit anti-phosphorylated (p)-c-Jun N-terminal Kinase (JNK) pAb (cat. no. 9251s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-JNK pAb (cat. no. 9252s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-p-p44/p42 pAb (cat. no. 9101s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-p44/p42 pAb (cat. no. 9102s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-p-Akt S473 mAb (cat. no. 4060s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-Akt mAb (cat. no. 2938s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-ALDH1A1 mAb (cat. no. ab52492; Abcam; 1:5,000); rabbit anti-ALDH1A3 pAb (cat. no. PA5-29188; Thermo Fisher Scientific, Inc.; 1:5,000); and mouse anti-β-actin mAb (cat. no. 60008-1-Ig; ProteinTech Group, Inc.; 1:10,000).

Techniques: Western Blot, Knockdown, Incubation, Cell Culture, Expressing, Transfection, Molecular Weight, Small Interfering RNA, Negative Control